Description
SNAP-8 10mg — Product Identity and Format
SNAP-8 10mg is a research-grade lyophilised preparation of SNAP-8 (Acetyl Octapeptide-3), a synthetic octapeptide analogue of the N-terminal sequence of SNAP-25 (synaptosomal-associated protein 25 kDa), a key component of the SNARE (soluble NSF attachment protein receptor) complex responsible for neurotransmitter exocytosis at the neuromuscular junction. The sequence of SNAP-8 is Ac-Glu-Glu-Met-Gln-Arg-Arg-Ala-Asp-NH₂ (Acetyl-EEMQRRAD-NH₂), corresponding to residues 12–19 of the SNAP-25 protein. Each 10mg vial supplied by Core Research contains lyophilised SNAP-8 acetate salt, verified by RP-HPLC to a minimum purity of ≥99.0%, with molecular identity confirmed by ESI-MS.
The SNARE complex is the core molecular machinery responsible for calcium-triggered neurotransmitter exocytosis at synaptic terminals and neuromuscular junctions. The complex is formed by the assembly of three SNARE proteins: SNAP-25 (on the presynaptic membrane), syntaxin-1 (on the presynaptic membrane), and VAMP/synaptobrevin (on the synaptic vesicle). Upon calcium influx triggered by an action potential, the SNARE complex zippers together, bringing the vesicle membrane into close apposition with the presynaptic membrane and driving membrane fusion and neurotransmitter release. SNAP-8 is proposed to compete with endogenous SNAP-25 for incorporation into the SNARE complex, thereby modulating the efficiency of SNARE complex assembly and vesicle fusion.
Compound Identity Matrix
| Attribute | Value | Specification |
|---|---|---|
| Common Name | SNAP-8; Acetyl Octapeptide-3 | Synthetic SNAP-25 N-terminal octapeptide analogue |
| Sequence | Ac-Glu-Glu-Met-Gln-Arg-Arg-Ala-Asp-NH₂ | 8 amino acids; N-Ac; C-terminal amide; SNAP-25 residues 12–19 |
| CAS Number | 868844-74-0 | SNAP-8 (Acetyl Octapeptide-3) |
| Molecular Formula | C41H70N14O16S | Free base |
| Molecular Weight | 1,075.15 Da | Free base (monoisotopic) |
| Molecular Target | SNARE complex / SNAP-25 | Competitive inhibitor of SNARE complex assembly |
| Physical Form | White lyophilised powder | Acetate salt; hygroscopic |
| Quantity per Vial | 10 mg | Research-grade lyophilised preparation |
| Purity (RP-HPLC) | ≥99.0% | C18 column, UV 220 nm |
| Identity Confirmation | ESI-MS | ±0.1 Da of theoretical MW |
Analytical Specification & Release Testing
Every batch of SNAP-8 10mg supplied by Core Research is manufactured under GMP-aligned conditions and subjected to a comprehensive analytical release protocol. Purity is determined by RP-HPLC (C18 column, UV 220 nm) confirming ≥99.0% purity with a maximum single impurity of ≤0.5%. Molecular identity is confirmed by ESI-MS with the observed [M+nH]ⁿ⁺ ion series matched against the theoretical mass of SNAP-8 (MW 1,075.15 Da) to within ±0.1 Da. Endotoxin content is confirmed at <1.0 EU/mg by LAL kinetic turbidimetric assay. Residual moisture is confirmed at <5.0% w/w by Karl Fischer titration.
| Test Parameter | Method | Specification | Typical Result |
|---|---|---|---|
| Purity (RP-HPLC) | C18 column, UV 220 nm | ≥99.0% | ≥99.5% |
| Identity (ESI-MS) | Electrospray ionisation MS | ±0.1 Da of theoretical MW | Conforms |
| Endotoxin (LAL) | Kinetic turbidimetric LAL | <1.0 EU/mg | <0.5 EU/mg |
| Residual Moisture | Karl Fischer titration | <5.0% w/w | <3.0% w/w |
| Appearance | Visual inspection | White lyophilised powder | Conforms |
SNARE Complex Research Targets
| Target / Component | Role in SNARE Complex | SNAP-8 Interaction |
|---|---|---|
| SNAP-25 | Presynaptic membrane t-SNARE; contributes two SNARE motifs | SNAP-8 mimics SNAP-25 N-terminal sequence (residues 12–19) |
| Syntaxin-1 | Presynaptic membrane t-SNARE; anchors SNARE complex | Competitive inhibition of SNAP-25/syntaxin-1 interaction |
| VAMP/Synaptobrevin | Vesicle v-SNARE; drives membrane fusion upon zippering | Indirect effect via reduced SNARE complex nucleation |
| Neuromuscular junction | Site of acetylcholine exocytosis; SNARE-dependent | Pre-clinical model for studying SNARE inhibition effects |
Storage, Stability & Handling
| Condition | Specification | Notes |
|---|---|---|
| Long-term storage (lyophilised) | −20°C | Stable for ≥24 months from manufacture date |
| Short-term storage (lyophilised) | 2–8°C | Acceptable for up to 4 weeks; desiccated |
| Reconstituted solution | 2–8°C, use within 14 days | Aliquot and freeze at −80°C for extended storage |
| Freeze-thaw cycles | Minimise; ≤3 cycles recommended | Aliquot prior to freezing |
| Humidity | Low humidity environment | Hygroscopic peptide; desiccant recommended |
Frequently Asked Questions
What is the molecular weight of SNAP-8?
The molecular weight of SNAP-8 (Acetyl Octapeptide-3) is 1,075.15 Da (free base). The molecular formula is C41H70N14O16S and the CAS number is 868844-74-0. The sequence is Ac-Glu-Glu-Met-Gln-Arg-Arg-Ala-Asp-NH₂.
Is a Certificate of Analysis available?
Yes. Batch-specific CoA documents — including annotated HPLC chromatograms and MS data — are available through the Core Research CoA Portal and are included with each order.
Related Research Guides
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Quality
Certificate of Analysis Portal
Category
Cosmetic Research Catalogue
Research context
The published literature on this material is summarised in the laboratory guides linked below. This record describes the material as supplied: identity, format, analytics, storage and documentation. It does not describe effects, and none should be inferred from the catalogue category.
For Research Use Only
SNAP-8 is supplied exclusively for in vitro laboratory and pre-clinical research. Not for human or veterinary use. No formulation, application, or usage guidance is provided. Purchase confirms the buyer is a qualified researcher.
