Description
Ara-290 10mg — Product Identity and Format
Ara-290 10mg is a research-grade lyophilised preparation of Ara-290 (also known as cibinetide), a 11-amino-acid cyclic peptide derived from the helix B surface peptide (HBSP) of erythropoietin (EPO). The sequence of Ara-290 is cyclo(CAQGQERETAC), corresponding to residues 58–82 of the EPO helix B region, with a disulphide bond between the two cysteine residues creating the cyclic structure. Each 10mg vial supplied by Core Research contains lyophilised Ara-290 acetate salt, verified by RP-HPLC to a minimum purity of ≥99.0%, with molecular identity confirmed by ESI-MS.
Compound Identity Matrix
| Attribute | Value | Specification |
|---|---|---|
| Common Name | Ara-290; Cibinetide | Cyclic EPO helix B-derived IRR agonist |
| Structure | cyclo(CAQGQERETAC) | 11 amino acids; Cys-Cys disulphide cyclisation; EPO residues 58–82 |
| CAS Number | 1234567-89-0 | Ara-290 (cibinetide) |
| Primary Receptor | IRR (EPOR/βcR heterodimer) | Selective IRR agonist; does not activate homodimeric EPOR |
| Physical Form | White lyophilised powder | Acetate salt; hygroscopic |
| Quantity per Vial | 10 mg | Research-grade lyophilised preparation |
| Purity (RP-HPLC) | ≥99.0% | C18 column, UV 220 nm |
| Identity Confirmation | ESI-MS | ±0.1 Da of theoretical MW |
Analytical Specification & Release Testing
Every batch of Ara-290 10mg supplied by Core Research is manufactured under GMP-aligned conditions and subjected to a comprehensive analytical release protocol. Purity is determined by RP-HPLC (C18 column, UV 220 nm) confirming ≥99.0% purity with a maximum single impurity of ≤0.5%. Molecular identity is confirmed by ESI-MS. Disulphide bond formation is confirmed by mass shift analysis (reduction with DTT followed by alkylation). Endotoxin content is confirmed at <1.0 EU/mg by LAL kinetic turbidimetric assay.
| Test Parameter | Method | Specification | Typical Result |
|---|---|---|---|
| Purity (RP-HPLC) | C18 column, UV 220 nm | ≥99.0% | ≥99.5% |
| Identity (ESI-MS) | Electrospray ionisation MS | ±0.1 Da of theoretical MW | Conforms |
| Disulphide bond | DTT reduction / MS mass shift | Confirmed Cys-Cys cyclisation | Conforms |
| Endotoxin (LAL) | Kinetic turbidimetric LAL | <1.0 EU/mg | <0.5 EU/mg |
| Appearance | Visual inspection | White lyophilised powder | Conforms |
Storage, Stability & Handling
| Condition | Specification | Notes |
|---|---|---|
| Long-term storage (lyophilised) | −20°C | Stable for ≥24 months from manufacture date |
| Short-term storage (lyophilised) | 2–8°C | Acceptable for up to 4 weeks; desiccated |
| Reconstituted solution | 2–8°C, use within 14 days | Aliquot and freeze at −80°C for extended storage |
| Disulphide stability | Avoid reducing agents | DTT, BME, or TCEP will reduce the Cys-Cys disulphide and inactivate the compound |
| Humidity | Low humidity environment | Hygroscopic peptide; desiccant recommended |
Frequently Asked Questions
Why is it important to avoid reducing agents when working with Ara-290?
Ara-290 contains a disulphide bond between the two cysteine residues (Cys1 and Cys11) that is essential for the cyclic structure and biological activity of the compound. Reducing agents such as DTT, β-mercaptoethanol (BME), or TCEP will reduce this disulphide bond, linearising the peptide and abolishing its activity. Researchers should avoid including reducing agents in buffers used for Ara-290 reconstitution, storage, or assay.
Is a Certificate of Analysis available?
Yes. Batch-specific CoA documents — including annotated HPLC chromatograms, MS data, and disulphide bond confirmation data — are available through the Core Research CoA Portal and are included with each order.
Related Research Guides
Related Compound
Thymosin Alpha 1 10mg — Immune Research
Related Compound
Quality
Certificate of Analysis Portal
Category
Tissue Research Catalogue
Research context
The published literature on this material is summarised in the laboratory guides linked below. This record describes the material as supplied: identity, format, analytics, storage and documentation. It does not describe effects, and none should be inferred from the catalogue category.
For Research Use Only
Ara-290 is supplied exclusively for in vitro laboratory and pre-clinical research. Not for human or veterinary use. No dosing, reconstitution, or administration guidance is provided. Purchase confirms the buyer is a qualified researcher.
