Description
Thymosin Alpha 1 (Tα1) 10mg — Product Identity and Format
Thymosin Alpha 1 (Tα1) 10mg is a research-grade lyophilised preparation of Thymosin Alpha 1, a 28-amino-acid peptide originally isolated from thymosin fraction 5 of bovine thymus by Allan Goldstein and colleagues in 1977. The sequence of Tα1 is Ac-Ser-Asp-Ala-Ala-Val-Asp-Thr-Ser-Ser-Glu-Ile-Thr-Thr-Lys-Asp-Leu-Lys-Glu-Lys-Lys-Glu-Val-Val-Glu-Glu-Ala-Glu-Asn-OH, incorporating an N-terminal acetylation that is essential for biological activity. Each 10mg vial supplied by Core Research contains lyophilised Tα1 acetate salt, verified by RP-HPLC to a minimum purity of ≥99.0%, with molecular identity confirmed by ESI-MS.
Compound Identity Matrix
| Attribute | Value | Specification |
|---|---|---|
| Common Name | Thymosin Alpha 1 (Tα1); Thymalfasin | 28-amino-acid thymic immunomodulatory peptide |
| Residue Count | 28 amino acids | N-terminal acetylation essential for activity |
| CAS Number | 62304-98-7 | Thymosin Alpha 1 (human) |
| Molecular Formula | C129H215N33O55 | Free base (N-terminal acetylated) |
| Molecular Weight | 3,108.28 Da | Free base (monoisotopic) |
| Primary Targets | TLR2, TLR9; T-cell receptors | Innate and adaptive immune system modulation |
| Physical Form | White lyophilised powder | Acetate salt; hygroscopic |
| Quantity per Vial | 10 mg | Research-grade lyophilised preparation |
| Purity (RP-HPLC) | ≥99.0% | C18 column, UV 220 nm |
| Identity Confirmation | ESI-MS | ±0.1 Da of theoretical MW |
Analytical Specification & Release Testing
Every batch of Thymosin Alpha 1 10mg supplied by Core Research is manufactured under GMP-aligned conditions and subjected to a comprehensive analytical release protocol. Purity is determined by RP-HPLC (C18 column, UV 220 nm) confirming ≥99.0% purity with a maximum single impurity of ≤0.5%. Molecular identity is confirmed by ESI-MS with the observed [M+nH]ⁿ⁺ ion series matched against the theoretical mass of Tα1 (MW 3,108.28 Da) to within ±0.1 Da. Endotoxin content is confirmed at <1.0 EU/mg by LAL kinetic turbidimetric assay. Residual moisture is confirmed at <5.0% w/w by Karl Fischer titration.
| Test Parameter | Method | Specification | Typical Result |
|---|---|---|---|
| Purity (RP-HPLC) | C18 column, UV 220 nm | ≥99.0% | ≥99.5% |
| Identity (ESI-MS) | Electrospray ionisation MS | ±0.1 Da of theoretical MW | Conforms |
| Endotoxin (LAL) | Kinetic turbidimetric LAL | <1.0 EU/mg | <0.5 EU/mg |
| Residual Moisture | Karl Fischer titration | <5.0% w/w | <3.0% w/w |
| Appearance | Visual inspection | White lyophilised powder | Conforms |
Storage, Stability & Handling
| Condition | Specification | Notes |
|---|---|---|
| Long-term storage (lyophilised) | −20°C | Stable for ≥24 months from manufacture date |
| Short-term storage (lyophilised) | 2–8°C | Acceptable for up to 4 weeks; desiccated |
| Reconstituted solution | 2–8°C, use within 14 days | Aliquot and freeze at −80°C for extended storage |
| Freeze-thaw cycles | Minimise; ≤3 cycles recommended | Aliquot prior to freezing |
| Humidity | Low humidity environment | Hygroscopic peptide; desiccant recommended |
Frequently Asked Questions
What is the molecular weight of Thymosin Alpha 1?
The molecular weight of Thymosin Alpha 1 is 3,108.28 Da (free base, N-terminal acetylated). The molecular formula is C129H215N33O55 and the CAS number is 62304-98-7. Tα1 is a 28-amino-acid peptide with an N-terminal acetylation that is essential for biological activity.
Is a Certificate of Analysis available?
Yes. Batch-specific CoA documents — including annotated HPLC chromatograms and MS data — are available through the Core Research CoA Portal and are included with each order.
Related Research Guides
Related Compound
Ara-290 10mg — EPO Receptor Research
Related Compound
Quality
Certificate of Analysis Portal
Category
Immune Research Catalogue
Research context
The published literature on this material is summarised in the laboratory guides linked below. This record describes the material as supplied: identity, format, analytics, storage and documentation. It does not describe effects, and none should be inferred from the catalogue category.
For Research Use Only
Thymosin Alpha 1 is supplied exclusively for in vitro laboratory and pre-clinical research. Not for human or veterinary use. No dosing, reconstitution, or administration guidance is provided. Purchase confirms the buyer is a qualified researcher.
