Description
MOTS-c 40mg — Product Identity and Format
MOTS-c 40mg is a research-grade lyophilised preparation of MOTS-c (Mitochondrial Open Reading Frame of the 12S rRNA-c), a 16-amino-acid peptide encoded within the mitochondrial genome — specifically within the 12S ribosomal RNA gene. Each vial supplied by Core Research contains 40mg of MOTS-c, verified by reverse-phase high-performance liquid chromatography (RP-HPLC) to a minimum purity of ≥99.0%, with identity confirmed by electrospray ionisation mass spectrometry (ESI-MS).
The sequence of MOTS-c is MRWQEMGYIFYPRKLR-NH₂ (16 amino acids, C-terminal amide), with a molecular weight of 2,174.6 Da (free base). MOTS-c is translated from a non-canonical open reading frame within the mitochondrial 12S rRNA gene using a modified genetic code, making it one of only a small number of peptides known to be encoded by the mitochondrial genome. Published research has examined its relationship with folate/purine metabolism, AMPK-associated signalling, mitochondrial-to-nuclear communication, and stress-responsive gene expression in cell and animal models. These findings are primarily preclinical and do not establish clinical efficacy, safety, or approved therapeutic use in humans.
Compound Identity
| Attribute | Value | Notes |
|---|---|---|
| Common Name | MOTS-c | Mitochondrial Open Reading Frame of the 12S rRNA-c |
| Sequence | MRWQEMGYIFYPRKLR-NH₂ | 16 amino acids, C-terminal amide |
| CAS Number | 1627580-64-6 | MOTS-c (human) |
| Molecular Formula | C101H152N28O22S | Free base |
| Molecular Weight | 2,174.6 Da | Free base (monoisotopic) |
| Genomic Origin | Mitochondrial 12S rRNA gene (MT-RNR1) | Non-canonical ORF, modified genetic code |
| AMPK-Associated Signalling | Via folate cycle inhibition and AICAR accumulation | AMPK is not a direct molecular binding target; activation is indirect via AICAR pathway |
| Downstream Associations | SIRT1, NRF2, FoxO1, mTOR | Downstream pathway associations in preclinical models; not validated direct binding targets |
| Salt Form | Acetate | Research-grade lyophilised preparation |
| Physical Form | White lyophilised powder | Research-grade lyophilised preparation |
Analytical Specification and Release Testing
Every batch of MOTS-c 40mg supplied by Core Research is subjected to a two-tier analytical release protocol. Purity is determined by RP-HPLC (C18 column, UV 220 nm) confirming ≥99.0% purity. Molecular identity is confirmed by ESI-MS with the observed [M+nH]ⁿ⁺ ion series matched against the theoretical mass of MOTS-c (MW 2,174.6 Da). Batch-specific results are documented in the Certificate of Analysis.
| Test Parameter | Method | Specification |
|---|---|---|
| Purity (RP-HPLC) | C18 column, UV 220 nm, area normalisation | ≥99.0% |
| Identity (ESI-MS) | Electrospray ionisation MS | ±0.1 Da of theoretical MW (2,174.6 Da) |
| Endotoxin (LAL) | Kinetic turbidimetric LAL | <1.0 EU/mg |
| Residual Moisture | Karl Fischer titration | <5.0% w/w |
| Appearance | Visual inspection | White lyophilised powder |
Analytical Note — Core Research Quality Team
MOTS-c is a 16-amino-acid peptide with MW 2,174.6 Da. ESI-MS identity confirmation uses multiply-charged ions ([M+2H]²⁺ at m/z ~1,088, [M+3H]³⁺ at m/z ~726) rather than singly-charged ions. The C-terminal amide modification (–NH₂) is confirmed in the MS spectrum as it reduces the molecular mass by 1 Da relative to the free acid form. Batch-specific CoA documents are available through the CoA Portal.
Storage and Transport
| Condition | Specification |
|---|---|
| Long-Term Storage (lyophilised, sealed) | −20°C, protected from light and moisture |
| Stability Period | Stated on the batch-specific CoA |
| Transit | Ambient; cold-chain packaging included |
Note: Reconstituted solution storage, solvent selection, working concentration, and stability period guidance are not provided, consistent with Core Research’s Research Use Only supply policy. Researchers should refer to their institutional SOPs and the published literature for handling reconstituted peptide solutions.
Batch Documentation
Every batch of MOTS-c 40mg dispatched by Core Research is accompanied by a batch-specific Certificate of Analysis confirming RP-HPLC purity (≥99.0%), ESI-MS identity, endotoxin result, and Karl Fischer moisture. CoA documents are available via the Certificate of Analysis Portal. For further information on our analytical methodology, refer to the guide on HPLC Purity in Peptide Research.
Frequently Asked Questions
What is the molecular weight and sequence of MOTS-c?
The molecular weight of MOTS-c is 2,174.6 Da (free base). The molecular formula is C₁₀₁H₁₅₂N₂₈O₂₂S and the CAS number is 1627580-64-6. The 16-amino-acid sequence is MRWQEMGYIFYPRKLR-NH₂ (C-terminal amide). Core Research supplies MOTS-c as the acetate salt form.
Is a Certificate of Analysis available?
Yes. Batch-specific CoA documents — including annotated HPLC chromatograms and MS data — are available through the Core Research CoA Portal and are included with each order.
Research context
The published literature on this material is summarised in the laboratory guides linked below. This record describes the material as supplied: identity, format, analytics, storage and documentation. It does not describe effects, and none should be inferred from the catalogue category.
For Research Use Only
MOTS-c is supplied exclusively for in vitro laboratory research. Not for human or veterinary use. No dosing, reconstitution, or administration guidance is provided.
