Description
NAD+ 1000mg — Product Identity and Format
NAD+ 1000mg is a research-grade preparation of nicotinamide adenine dinucleotide (NAD+), the oxidised form of the essential coenzyme that participates in over 500 enzymatic reactions in human metabolism. NAD+ serves as a hydride acceptor in catabolic reactions (glycolysis, the TCA cycle, and beta-oxidation), a substrate for NAD+-consuming enzymes including sirtuins (SIRT1–7), poly(ADP-ribose) polymerases (PARPs), and CD38/CD157 ectoenzymes, and a precursor for cyclic ADP-ribose (cADPR) — a second messenger involved in calcium signalling. Each vial supplied by Core Research contains 1000mg of NAD+ (free acid form), verified by HPLC to ≥99.0% purity with identity confirmed by UV spectroscopy (λmax 260 nm) and LC-MS.
NAD+ is a dinucleotide coenzyme, not a peptide.
Compound Identity
| Attribute | Value | Notes |
|---|---|---|
| Full Name | Nicotinamide Adenine Dinucleotide (oxidised) | Free acid form |
| Abbreviation | NAD+, NAD, DPN | Diphosphopyridine nucleotide (historical) |
| CAS Number | 53-84-9 | NAD+ free acid |
| Molecular Formula | C21H27N7O14P2 | Free acid |
| Molecular Weight | 663.4 Da | Free acid |
| UV Absorbance | λmax 260 nm (ε = 18,000 M⁻¹cm⁻¹) | Adenine chromophore; used for quantification |
| Primary Functions | Redox coenzyme, sirtuin substrate, PARP substrate | 500+ enzymatic reactions |
| Key Consuming Enzymes | SIRT1–7, PARP1/2, CD38, NAMPT | NAD+ is cleaved, not reduced, by these enzymes |
| Physical Form | White to off-white lyophilised powder | Free acid; hygroscopic |
Analytical Specification and Release Testing
| Test Parameter | Method | Specification |
|---|---|---|
| Purity (HPLC) | C18 column, UV 260 nm, area normalisation | ≥99.0% |
| Identity (LC-MS) | LC-MS/MS | m/z 664.1 [M+H]+ — conforms |
| UV Absorbance | UV spectroscopy | λmax 260 nm — conforms |
| Residual Moisture | Karl Fischer titration | <5.0% w/w |
| Appearance | Visual inspection | White to off-white powder — conforms |
Analytical Note — Core Research Quality Team
NAD+ is characterised by HPLC with UV detection at 260 nm, exploiting the adenine chromophore (ε = 18,000 M⁻¹cm⁻¹). This wavelength is specific to the adenine ring system and distinguishes NAD+ from nicotinamide mononucleotide (NMN) and nicotinamide riboside (NR), which lack the adenine chromophore at this wavelength. LC-MS identity confirmation uses the [M+H]⁺ ion at m/z 664.1, consistent with the free acid molecular weight of 663.4 Da. Batch-specific CoA documents are available through the CoA Portal.
Sirtuin Biochemistry and Enzyme Kinetics
NAD+ is the essential co-substrate for all seven mammalian sirtuins (SIRT1–7). In vitro sirtuin activity assays — including fluorometric SIRT1 activity assays, SIRT3 deacetylase assays, and SIRT6 deacylase assays — require NAD+ as a co-substrate at defined concentrations. Core Research’s NAD+ 1000mg is suitable for use in sirtuin biochemistry research, enzyme kinetics studies, and high-throughput screening assays for sirtuin modulators.
Storage and Transport
| Condition | Specification |
|---|---|
| Long-Term Storage (lyophilised, sealed) | −20°C, desiccated, protected from light |
| Stability Period | Stated on the batch-specific CoA |
| Transit | Ambient; cold-chain packaging included |
Note: Aqueous solution stability, solubility, pH sensitivity, and reconstitution guidance are not provided, consistent with Core Research’s Research Use Only supply policy. NAD+ is hygroscopic and susceptible to hydrolysis in solution; researchers should refer to their institutional SOPs for handling dissolved preparations. Stability period is stated on the batch-specific CoA.
Batch Documentation
Every batch of NAD+ 1000mg dispatched by Core Research is accompanied by a batch-specific Certificate of Analysis confirming HPLC purity (≥99.0%), LC-MS identity, UV absorbance, and Karl Fischer moisture. CoA documents are available via the Certificate of Analysis Portal. For further information on our analytical methodology, refer to the guide on HPLC Purity in Research Compound Testing.
Frequently Asked Questions
Is a Certificate of Analysis available?
Yes. Batch-specific CoA documents — including annotated HPLC chromatograms and LC-MS data — are available through the Core Research CoA Portal and are included with each order.
Research context
The published literature on this material is summarised in the laboratory guides linked below. This record describes the material as supplied: identity, format, analytics, storage and documentation. It does not describe effects, and none should be inferred from the catalogue category.
For Research Use Only
NAD+ is supplied exclusively for in vitro laboratory research. Not for human or veterinary use. No dosing, administration, or reconstitution guidance is provided.
