Description
NAD+ Refill 1000mg — β-Nicotinamide Adenine Dinucleotide Research-Grade Refill Cartridge for the Core Research Peptide Pen v3
The NAD+ Refill 1000mg is a pre-filled refill cartridge containing research-grade β-nicotinamide adenine dinucleotide (NAD+, oxidised form), the essential redox coenzyme and signalling molecule that serves as a substrate for sirtuins (SIRT1–7), PARP enzymes, and CD38/CD157 NADases. This refill format is designed for direct use with the Core Research Reusable Metal Peptide Pen v3. Each refill cartridge contains 1000mg of NAD+ (free acid), verified by HPLC to a minimum purity of ≥99.0%, with molecular identity confirmed by ESI-MS.
NAD+ is a dinucleotide consisting of adenosine monophosphate (AMP) and nicotinamide mononucleotide (NMN) linked by a pyrophosphate bond.
The 1000mg refill format provides a substantial quantity for research programmes with ongoing NAD+ requirements, offering a cost-effective and convenient alternative to repeated small-volume purchases.
Compound Identity & Molecular Data
| Parameter | Value |
|---|---|
| Compound Name | β-NAD+ (β-Nicotinamide adenine dinucleotide, oxidised form) |
| Molecular Formula | C₂₁H₂₇N₇O₁₄P₂ |
| Molecular Weight | 663.43 Da (free acid, anhydrous) |
| CAS Number | 53-84-9 |
| Form | Free acid (not sodium salt); research-grade preparation |
| Primary Enzyme Substrates | SIRT1–7 (sirtuins), PARP1/2, CD38, CD157, SARM1 |
| Redox Function | NAD+/NADH cycling in glycolysis, TCA cycle, ETC |
| Research Classification | Redox coenzyme; sirtuin substrate; PARP substrate; ageing & metabolism research |
Analytical Release Testing — Refill Cartridge Specification
| Test | Method | Specification | Notes |
|---|---|---|---|
| Purity | HPLC (UV 260 nm) | ≥99.0% | Reverse-phase HPLC; UV detection at 260 nm (adenine absorbance) |
| Identity | ESI-MS | Confirmed ±0.1 Da | Expected [M+H]⁺: 664.44 |
| Endotoxin | Kinetic turbidimetric LAL | <1.0 EU/mg | USP <85> compliant method |
| Residual Moisture | Karl Fischer titration | <5.0% | Coulometric KF; critical for NAD+ stability |
| Quantity per Cartridge | Gravimetric | 1000 mg ± 5% | Free acid; net NAD+ content |
| Compatible Device | — | Core Research Peptide Pen v3 | Use with 4mm 31G pen needles |
Reconstitution & Handling Guidance
NAD+ is supplied as a dry powder in the refill cartridge and must be dissolved before use. For in vivo research, dissolve in sterile saline (0.9% NaCl) or PBS (pH 7.4) immediately before use, as NAD+ hydrolyses in aqueous solution. Prepare fresh solutions for each experiment; do not store dissolved NAD+ solutions for extended periods.
Storage & Stability
| Condition | Temperature | Duration | Notes |
|---|---|---|---|
| Dry powder (cartridge, long-term) | −20°C | ≥24 months | Desiccated; inert atmosphere; protect from light and moisture |
| Dry powder (cartridge, short-term) | 2–8°C | Up to 3 months | Desiccated; inert atmosphere; suitable for active research use |
| Dissolved solution | Prepare fresh; −80°C for aliquots | Use within 24h or freeze immediately | NAD+ hydrolyses in aqueous solution; prepare fresh or store at −80°C under inert atmosphere |
Frequently Asked Questions
Why does NAD+ need to be prepared fresh in aqueous solution?
NAD+ undergoes hydrolysis of the pyrophosphate bond in aqueous solution, particularly at acidic or alkaline pH and elevated temperatures. The rate of hydrolysis is significant at room temperature and increases substantially above pH 8 or below pH 5. For research applications requiring stable NAD+ solutions, prepare fresh from dry powder immediately before use, or store as single-use aliquots at −80°C under inert atmosphere.
What is the difference between NAD+ free acid and NAD+ sodium salt?
NAD+ free acid (CAS 53-84-9) and NAD+ disodium salt (CAS 606-68-8) are both used in research, but differ in solubility and pH effects. The free acid form dissolves to give a slightly acidic solution; the disodium salt dissolves to give a near-neutral solution. For most research applications, either form is suitable, but the free acid form is preferred when precise pH control is required. The Core Research NAD+ Refill is supplied as the free acid form.
Is a Certificate of Analysis provided with each refill?
Yes. A batch-specific Certificate of Analysis documenting HPLC purity, ESI-MS identity, LAL endotoxin result, and Karl Fischer moisture content is included with every NAD+ Refill 1000mg order.
Related Research Guides
Standard Format
NAD+ 1000mg — Full Vial
Related Refill
MOTS-c Refill 40mg
Compatible Device
Reusable Metal Peptide Pen v3
Reconstitution
Bacteriostatic Water 10ml
Pen Needles
GlucoRx Pen Needles 4mm 31G
Category
All Refill Cartridges
Research context
The published literature on this material is summarised in the laboratory guides linked below. This record describes the material as supplied: identity, format, analytics, storage and documentation. It does not describe effects, and none should be inferred from the catalogue category.
For Research Use Only — Not for Human or Veterinary Use
NAD+ Refill 1000mg is supplied exclusively for in vitro and pre-clinical in vivo research use with the Core Research Reusable Metal Peptide Pen v3. This product has not been evaluated by the MHRA, FDA, or any other regulatory authority for safety or efficacy in humans or animals. Purchase confirms the buyer is a qualified researcher.
