Description
BPC-157 Refill 10mg — Research-Grade Pentadecapeptide Refill Cartridge for the Core Research Peptide Pen v3
The BPC-157 Refill 10mg is a pre-filled lyophilised refill cartridge containing research-grade BPC-157 (Body Protection Compound-157), a synthetic pentadecapeptide with the sequence Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val (15 amino acids), derived from a gastroprotective region of the human gastric juice protein BPC. This refill format fits the Core Research Reusable Metal Peptide Pen v3 and is supplied for laboratory dispensing workflows that have adopted the pen system; it is not an administration device.
Each refill cartridge contains 10mg of lyophilised BPC-157 acetate salt, verified by reverse-phase high-performance liquid chromatography (RP-HPLC) to a minimum purity of ≥99.0%, with molecular identity confirmed by electrospray ionisation mass spectrometry (ESI-MS). Endotoxin content is tested by kinetic turbidimetric Limulus Amebocyte Lysate (LAL) assay and certified at <1.0 EU/mg. Residual moisture is verified by Karl Fischer titration at <5.0%. A batch-specific Certificate of Analysis (CoA) is included with every order.
BPC-157 was first characterised by Professor Predrag Sikiric and colleagues at the University of Zagreb, where it was isolated as the active fragment of a larger gastric juice protein.
Compound Identity & Molecular Data
| Parameter | Value |
|---|---|
| Compound Name | BPC-157 (Body Protection Compound-157) |
| Sequence | Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val |
| Residue Count | 15 amino acids (pentadecapeptide) |
| Molecular Formula | C₆₂H₉₈N₁₆O₂₂ |
| Molecular Weight | 1,419.55 Da (free base, monoisotopic) |
| CAS Number | 137525-51-0 |
| Salt Form | Acetate salt (lyophilised) |
| Structural Features | Linear; no disulphide bonds; proline-rich N-terminal region |
| Primary Targets | VEGFR2, FAK/paxillin, eNOS/nNOS, GHR/JAK2 |
| Research Classification | Tissue repair peptide; gastroprotective; angiogenic |
Analytical Release Testing — Refill Cartridge Specification
| Test | Method | Specification | Notes |
|---|---|---|---|
| Purity | RP-HPLC (C18, UV 220 nm) | ≥99.0% | Acetonitrile/water gradient; TFA modifier |
| Identity | ESI-MS | Confirmed ±0.1 Da | Expected [M+H]⁺: 1,420.56; [M+2H]²⁺: 710.78 |
| Endotoxin | Kinetic turbidimetric LAL | <1.0 EU/mg | Chromogenic LAL assay; USP <85> compliant method |
| Residual Moisture | Karl Fischer titration | <5.0% | Coulometric KF; ensures lyophilisation quality |
| Quantity per Cartridge | Gravimetric | 10 mg ± 5% | Lyophilised acetate salt; net peptide content |
| Compatible Device | — | Core Research Peptide Pen v3 | Use with 4mm 31G pen needles; bacteriostatic water for reconstitution |
Refill Cartridge vs Full Vial — Choosing the Right Format
The BPC-157 Refill 10mg is made for the Core Research Reusable Metal Peptide Pen v3 and is intended for laboratories that have already adopted the pen system for dispensing. Compared with the standard vial, the pre-filled cartridge removes repeated vial reconstitution and syringe loading from the workflow, which reduces contamination risk and volume variability between aliquots, and the graduated dial sets a consistent dispensed volume across replicate preparations and time points.
Researchers who have not adopted the pen system, or who require BPC-157 for in vitro assays or cell culture experiments, should consider the BPC-157 10mg standard vial format, which provides the same research-grade material in a conventional lyophilised vial suitable for reconstitution in any appropriate buffer. The BPC-157 / TB-500 Blend 10mg supplies the two peptides as a single co-lyophilised preparation.
Reconstitution & Handling Guidance
BPC-157 Refill cartridges are supplied in lyophilised form and must be reconstituted before use. For use with the Core Research Peptide Pen v3, reconstitute the cartridge with bacteriostatic water (0.9% benzyl alcohol in sterile water for injection) to the desired concentration. Allow the lyophilised cake to dissolve completely by gentle swirling — do not vortex or shake vigorously, as mechanical agitation can cause peptide aggregation. The reconstituted solution should be clear and colourless; discard if particulate matter or discolouration is observed.
Working solutions should be prepared fresh from frozen aliquots where possible. BPC-157 is soluble in aqueous buffers at concentrations up to 10mg/ml under standard conditions; higher concentrations may require brief sonication or gentle warming to 37°C to achieve complete dissolution.
Storage & Stability
| Condition | Temperature | Duration | Notes |
|---|---|---|---|
| Lyophilised cartridge (long-term) | −20°C | ≥24 months | Desiccated; protect from light and moisture |
| Lyophilised cartridge (short-term) | 2–8°C | Up to 4 weeks | Desiccated; suitable for active research use |
| Reconstituted in bacteriostatic water | 2–8°C | Up to 28 days | Benzyl alcohol preservative extends stability; in-pen use |
| Reconstituted aliquots (extended) | −80°C | Up to 12 months | Single-use aliquots; avoid repeated freeze-thaw cycles |
Frequently Asked Questions
Is the BPC-157 Refill compatible with all versions of the Core Research Peptide Pen?
The BPC-157 Refill 10mg is designed specifically for the Core Research Reusable Metal Peptide Pen v3. It is not guaranteed to be compatible with earlier pen versions or third-party pen devices. Researchers using other delivery systems should use the standard BPC-157 10mg vial format.
What volume of bacteriostatic water should be used to reconstitute the refill?
The working concentration is a property of the assay rather than of the cartridge; the reconstitution calculator and the handling guides cover concentration and volume for laboratory preparation.
How does the BPC-157 Refill differ from the BPC-157 / TB-500 Blend Refill?
The BPC-157 Refill 10mg contains only BPC-157, allowing researchers to study the effects of BPC-157 in isolation. The BPC-157 / TB-500 Blend Refill contains both BPC-157 and TB-500 in a defined ratio, for research into the combined or synergistic effects of both peptides.
Is a Certificate of Analysis provided with each refill?
Yes. A batch-specific Certificate of Analysis documenting RP-HPLC purity, ESI-MS identity confirmation, LAL endotoxin result, and Karl Fischer moisture content is included with every BPC-157 Refill 10mg order. The CoA is specific to the manufacturing batch supplied and is available for download from your order confirmation.
Related Research Guides
Standard Format
BPC-157 10mg — Full Vial
Blend Format
BPC-157 / TB-500 Blend 10mg
Complementary Refill
TB-500 Refill 10mg
Compatible Device
Reusable Metal Peptide Pen v3
Reconstitution
Bacteriostatic Water 10ml
Category
All Refill Cartridges
Research context
The published literature on this material is summarised in the laboratory guides linked below. This record describes the material as supplied: identity, format, analytics, storage and documentation. It does not describe effects, and none should be inferred from the catalogue category.
For Research Use Only — Not for Human or Veterinary Use
BPC-157 Refill 10mg is supplied exclusively for in vitro and pre-clinical in vivo research use with the Core Research Reusable Metal Peptide Pen v3. This product has not been evaluated by the MHRA, FDA, or any other regulatory authority for safety or efficacy in humans or animals. Purchase and use of this product confirms that the buyer is a qualified researcher operating within applicable institutional and legal frameworks.
